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KMID : 0360419940300020167
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Korean Journal of Pharmacology
1994 Volume.30 No. 2 p.167 ~ p.179
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Effects of TGF β_(1) on the Growth and Biochemical Changes in Cultured Rat Glial Cells
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Abstract
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Recent evidence indicates that glial cells have a wide range of funtions which are critical for maintaining a balanced homeostatic environment in the central nervous system (CNS) peripheral nervous system(PNS). Morever, astrocytes are known to participate in the tissue repair and neuroimmunologic events within the CNS through many kinds of growth factors and ´cytokines. We investigated the effect of TGF Ai on the growth and biochemical changes of rat glial cells in culture. The proliferative effect was determined by 3H-thymidine uptake and the double immunostain with anti-cell-specific- marker and anti-Bromodeoxyuridine(BrdU) antibody. To check the effect of biochemical changes we compared the amounts of glial fibrillar acidic protein(GFAP) and the activity of glutamine synthetase(GS) in astrocyte. And the amounts of myelin basic protein and the activity of 2´,3´-cyclic nucleotide phosphohydrolase(CNPase) were measured in oligodendrocyte and the .amounts of peripheral´ myelin in Schwann cell. When TGF was treated for 2 days with cultured glial cell, TGF j9 decreased the 3H-thymidine uptake and proliferation index of double immunostain of astrocytes, which .indicates the inhibition of astroglial DNA synthesis, but stimulated the growth of Schwann cell. Also, TGF /3, decrease the GS activity and in-creased the amounts of GFAP in astrocyte. In the case of Schwann cells the amounts of peripheral myelin was increased when treated with TGF A. However, TGF /3; didn´t show any effect on the proliferation and biochemical changes in oligodendrocyte. These results suggest that TGF s9 might have a critical action in the regulation of proliferation and biochemical changes in glial cells, especially astrocyte.
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